Revista Fitos
A composição fitoquímica e o potencial anti-inflamatório in vitro de produtos de moringa - amostra padronizada de extrato de folha rico em flavonoides obtida por processo de produção otimizado (cultivo, colheita e extração) e seis amostras comerciais de extrato ou pó de folha seca foram analisados. O conteúdo fenólico total, perfil químico (TLC e HPLC), vitexina, efeito anti-inflamatório in vitro em células RAW 264.7 (macrófagos murinos) foram determinados e comparados. O extrato padronizado apresentou maior teor de fenólicos e perfil cromatográfico mais complexo que amostras comerciais, além de atividade anti-inflamatória e supressão de óxido nítrico mais potentes, regulação negativa da expressão dos genes pró-inflamatórios iNOS e TNF-α e regulação positiva do gene IL-10 a 50 μg/mL. Os extratos apresentaram melhores resultados que as amostras de folhas em pó. Essas descobertas demonstram a importância da padronização para obter produtos de moringa confiáveis para farmacêuticos, cosméticos ou suplementos.
DOI
10.32712/2446-4775.2025.1724
Autores
Identificação
Referências do artigo
1. Castro FD, Silva LMP, Oliveira RB, Zucolotto SM. Potencial efeito terapêutico das folhas de Moringa
oleifera Lamarck (Moringaceae): uma revisão do seu papel no controle da glicemia em estudos in vivo. Rev
Fitos [Internet]. 2022 Dec 20; 16(4): 508–540. Available from:
[https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/1315].
2. Gharsallah K, Rezig L, Rajoka MSR, Mehwish HM, Ali MA, Chew SC. Moringa oleifera: Processing,
phytochemical composition, and industrial applications. South African J Bot. [Internet]. 2023 Sep; 160:
180–193. Available from: [https://linkinghub.elsevier.com/retrieve/pii/S0254629923003873].
3. Leone A, Spada A, Battezzati A, Schiraldi A, Aristil J, Bertoli S. Cultivation, Genetic, Ethnopharmacology,
Phytochemistry and Pharmacology of Moringa oleifera Leaves: An Overview. Int J Mol Sci. [Internet]. 2015
Jun 5; 16(12): 12791–12835. Available from: [http://www.mdpi.com/1422-0067/16/6/12791].
4. Singh PA, Bajwa N, Chinnam S, Chandan A, Baldi A. An overview of some important deliberations to
promote medicinal plants cultivation. J Appl Res Med Aromat Plants [Internet]. 2022 Dec; 31: 100400.
Available from: [https://linkinghub.elsevier.com/retrieve/pii/S2214786122000328].
5. Yang M, Tao L, Kang X-R, Wang Z-L, Su L-Y, Li L-F, et al. Moringa oleifera Lam. leaves as new raw food
material: A review of its nutritional composition, functional properties, and comprehensive application.
Trends Food Sci Technol. [Internet]. 2023 Aug; 138: 399–416. Available from:
[https://linkinghub.elsevier.com/retrieve/pii/S0924224423001577]
6. Mukherjee PK, Bahadur S, Chaudhary SK, Kar A, Mukherjee K. Quality Related Safety Issue-EvidenceBased Validation of Herbal Medicine Farm to Pharma [Internet]. In: Evidence-Based Validation of Herbal
Medicine. Elsevier; 2015. p. 1–28. Available from:
[https://linkinghub.elsevier.com/retrieve/pii/B9780128008744000015]
7. Fibigr J, Šatínský D, Solich P. Current trends in the analysis and quality control of food supplements based
on plant extracts. Anal Chim Acta [Internet]. 2018 Dec; 1036: 1–15. Available from:
[https://linkinghub.elsevier.com/retrieve/pii/S0003267018309541]
8. Palhares RM, Baratto LC, Scopel M, Mügge FLB, Brandão MGL. Medicinal Plants and Herbal Products
from Brazil: How Can We Improve Quality? Front Pharmacol. [Internet]. 2021 Jan 27; 11. Available from:
[https://www.frontiersin.org/articles/10.3389/fphar.2020.606623/full].
9. Chokwe RC, Dube S, Nindi MM. Development of an HPLC-DAD Method for the Quantification of Ten
Compounds from Moringa oleifera Lam. and Its Application in Quality Control of Commercial Products.
Molecules [Internet]. 2020 Sep 28; 25(19): 4451. Available from: [https://www.mdpi.com/1420-
3049/25/19/4451]
10. Fejér J, Kron I, Pellizzeri V, Pľuchtová M, Eliašová A, Campone L, et al. First Report on Evaluation of
Basic Nutritional and Antioxidant Properties of Moringa Oleifera Lam. from Caribbean Island of Saint Lucia.
Plants [Internet]. 2019 Nov 23; 8(12): 537. Available from: [https://www.mdpi.com/2223-7747/8/12/537].
11. Stohs SJ, Hartman MJ. Review of the Safety and Efficacy of Moringa oleifera. Phyther Res. [Internet].
2015 Jun 24; 29(6): 796–804. Available from: [https://onlinelibrary.wiley.com/doi/10.1002/ptr.5325].
12. Ademiluyi AO, Aladeselu OH, Oboh G, Boligon AA. Drying alters the phenolic constituents, antioxidant
properties, α‐amylase, and α‐glucosidase inhibitory properties of Moringa (Moringa oleifera) leaf. Food Sci
Nutr [Internet]. 2018 Nov 10; 6(8): 2123–2133. Available from:
[https://onlinelibrary.wiley.com/doi/10.1002/fsn3.770].
13. Debelo H, Fiecke C, Terekhov A, Reuhs B, Hamaker B, Ferruzzi MG. Compositional analysis of
phytochemicals and polysaccharides from Senegalese plant ingredients: Adansonia digitata (baobab), Moringa oleifera (moringa) and Hibsicus sabdariffa (hibiscus). NFS J [Internet]. 2023 Aug; 32: 100144.
Available from: [https://linkinghub.elsevier.com/retrieve/pii/S2352364623000238].
14. Silva LMP, Inácio MRC, Silva GGC, Silva JMS, Luz JRD, Almeida MG, et al. The First Optimization
Process from Cultivation to Flavonoid-Rich Extract from Moringa oleifera Lam. Leaves in Brazil. Foods
[Internet]. 2022 May 17; 11(10): 1452. Available from: [https://www.mdpi.com/2304-8158/11/10/1452].
15. Hoskin RT, Xiong J, Esposito DA, Lila MA. Blueberry polyphenol-protein food ingredients: The impact of
spray drying on the in vitro antioxidant activity, anti-inflammatory markers, glucose metabolism and fibroblast
migration. Food Chem. [Internet]. 2019 May; 280: 187–194. Available from:
[https://linkinghub.elsevier.com/retrieve/pii/S0308814618321502].
16. Esposito D, Chen A, Grace MH, Komarnytsky S, Lila MA. Inhibitory Effects of Wild Blueberry
Anthocyanins and Other Flavonoids on Biomarkers of Acute and Chronic Inflammation in vitro. J Agric Food
Chem. [Internet]. 2014 Jul 23; 62(29): 7022–7028. Available from:
[https://pubs.acs.org/doi/10.1021/jf4051599].
17. Luetragoon T, Pankla Sranujit R, Noysang C, Thongsri Y, Potup P, Suphrom N, et al. Bioactive
Compounds in Moringa oleifera Lam. Leaves Inhibit the Pro-Inflammatory Mediators in LipopolysaccharideInduced Human Monocyte-Derived Macrophages. Molecules [Internet]. 2020 Jan 2; 25(1): 191. Available
from: [https://www.mdpi.com/1420-3049/25/1/191].
18. Choi S-Y, Hwang J-H, Ko H-C, Park J-G, Kim S-J. Nobiletin from citrus fruit peel inhibits the DNA-binding
activity of NF-κB and ROS production in LPS-activated RAW 264.7 cells. J Ethnopharmacol. [Internet].
2007 Aug; 113(1): 149–155. Available from:
[https://linkinghub.elsevier.com/retrieve/pii/S037887410700267X].
19. Nobossé P, Fombang EN, Mbofung CMF. Effects of age and extraction solvent on phytochemical content
and antioxidant activity of fresh Moringa oleifera L. leaves. Food Sci Nutr. [Internet]. 2018 Nov 14; 6(8):
2188–2198. Available from: [https://onlinelibrary.wiley.com/doi/10.1002/fsn3.783].
20. İlhan Dincer E, Temiz H. Investigation of physicochemical, microstructure and antioxidant properties of
firethorn (Pyracantha coccinea var. lalandi) microcapsules produced by spray-dried and freeze-dried
methods. South African J Bot. [Internet]. 2023 Apr; 155: 340–354. Available from:
[https://linkinghub.elsevier.com/retrieve/pii/S0254629923000844].
21. Rodríguez-Pérez C, Quirantes-Piné R, Fernández-Gutiérrez A, Segura-Carretero A. Optimization of
extraction method to obtain a phenolic compounds-rich extract from Moringa oleifera Lam leaves. Ind Crops
Prod. [Internet]. 2015 Apr; 66: 246–254. Available from:
[https://linkinghub.elsevier.com/retrieve/pii/S0926669015000035].
22. Kashyap P, Kumar S, Riar CS, Jindal N, Baniwal P, Guiné RPF, et al. Recent Advances in Drumstick
(Moringa oleifera) Leaves Bioactive Compounds: Composition, Health Benefits, Bioaccessibility, and Dietary
Applications. Antioxidants [Internet]. 2022 Feb 16; 11(2): 402. Available from: [https://www.mdpi.com/2076-
3921/11/2/402].
23. Wagner H, Bladt S. Plant drug analysis: a thin layer chromatography atlas [Internet]. 2nd ed. Berlin,
Heidelberg: Springer Science & Business Media; 1996. Available from:
[http://link.springer.com/10.1007/978-3-642-00574-9].
24. Lin M, Zhang J, Chen X. Bioactive flavonoids in Moringa oleifera and their health-promoting properties.
J Funct Foods [Internet]. 2018 Aug; 47: 469–479. Available from:
[https://linkinghub.elsevier.com/retrieve/pii/S1756464618303074].
25. Costa GM, Gazola AC, Madóglio FA, Zucolotto SM, Reginatto FH, Castellanos L, et al. Vitexin derivatives
as chemical markers in the differentiation of the closely related species passiflora alata curtis and Passiflora quadrangularis linn. J Liq Chromatogr Relat Technol. [Internet]. 2013 Apr 24; 36(12): 1697–1707.
Available from: [https://www.tandfonline.com/doi/full/10.1080/10826076.2012.695316].
26. Karthivashan G, Tangestani Fard M, Arulselvan P, Abas F, Fakurazi S. Identification of Bioactive
Candidate Compounds Responsible for Oxidative Challenge from Hydro‐Ethanolic Extract of Moringa
oleifera Leaves. J Food Sci. [Internet]. 2013 Sep 23; 78(9). Available from:
[https://ift.onlinelibrary.wiley.com/doi/10.1111/1750-3841.12233].
27. Klein-Junior LC, Souza MR, Viaene J, Bresolin TMB, Gasper AL, Henriques AT, et al. Quality Control of
Herbal Medicines: From Traditional Techniques to State-of-the-art Approaches. Pl Med. [Internet]. 2021 Oct
19; 87(12/13): 964–988. Available from: [http://www.thieme-connect.de/DOI/DOI?10.1055/a-1529-8339].
28. Peñalver R, Martínez-Zamora L, Lorenzo JM, Ros G, Nieto G. Nutritional and Antioxidant Properties of
Moringa oleifera Leaves in Functional Foods. Foods [Internet]. 2022 Apr 12; 11(8): 1107. Available from:
[https://www.mdpi.com/2304-8158/11/8/1107].
29. Fard M, Arulselvan P, Karthivashan G, Adam S, Fakurazi S. Bioactive extract from Moringa oleifera
inhibits the pro-inflammatory mediators in lipopolysaccharide stimulated macrophages. Pharmacogn Mag.
[Internet]. 2015; 11(44): 556. Available from: [https://phcog.com/article/view/2015/11/44s3/s556-s563]
Página da publicação
Publicado por (Instituto)