Visa em Debate
Introdução: A exposição humana a microplásticos de poliestireno (PS-MPs) pela dieta é uma preocupação crescente, porque seus efeitos no epitélio intestinal, incluindo estresse oxidativo e genotoxicidade, permanecem controversos. Objetivo: Avaliar e sintetizar evidências in vitro sobre esses desfechos em linhagens celulares de cólon humano. Método: Revisão sistemática conforme PRISMA, com buscas em PubMed, Scopus e Web of Science, resultando em dez estudos incluídos. Resultados: Os PS-MPs associaram-se a respostas de estresse celular, com aumento de espécies reativas de oxigênio e com modulação de marcadores antioxidantes e parâmetros mitocondriais. Evidências de genotoxicidade concentraram-se em estudos com partículas funcionalizadas (carboxiladas), enquanto exposições com partículas prístinas frequentemente não mostraram dano significativo ao DNA. Conclusões: A síntese indica perturbação redox em células intestinais expostas a PS-MPs, enquanto a genotoxicidade não se apresentou como efeito consistente nas condições avaliadas, portanto permanecendo dependente de características da partícula e do desenho analítico.
DOI
10.22239/2317-269X.02552
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1. Pilapitiya, PNT, Ratnayake, A.S. The world of plastic waste: a review. Clean Mat. 2024;11:1-23.https://doi.org/10.1016/j.clema.2024.100220
2. Ramsperger AFRM, Bergamaschi E, Panizzolo M, Fenoglio I, Barbero F, Peters R et al. Nano- and microplastics: a comprehensive review on their exposure routes, translocation, and fate in humans. NanoImpact. 2023;29:1-20.https://doi.org/10.1016/j.impact.2022.100441
3. Kurniawan TA, Mohyuddin A, Othman MHD, Goh HH, Zhang D, Anouzla A et al. Beyond surface: unveiling ecological and economic ramifications of microplastic pollution in the oceans. Water Environ Res. 2024;96(7).https://doi.org/10.1002/wer.11070
4. Amiri H, Hoseini M, Abbasi S, Malakootian M, Hashemi M, Jaafarzadeh N et al. Geophagy and microplastic ingestion. J Food Compos Anal. 2022;106.https://doi.org/10.1016/j.jfca.2021.104290
5. Schwabl P, Köppel S, Königshofer P, Bucsics T, Trauner M, Reiberger T et al. Detection of various microplastics in human stool: a prospective case series. Ann Intern Med. 2019;171(7):453-7.https://doi.org/10.7326/M19-0618
6. Barceló D, Picó Y, Alfarhan AH. Microplastics: detection in human samples, cell line studies, and health impacts. Environ Toxicol Pharmacol. 2023;101:1-16.https://doi.org/10.1016/j.etap.2023.104204
7. Cox KD, Covernton GA, Davies HL, Dower JF, Juanes F, Dudas SE. Human consumption of microplastics. Environ Sci Technol.2019;53(12):7068-74.https://doi.org/10.1021/acs.est.9b01517
8. Haddad MJ, Sztupecki W, Delayre-Orthez C, Rhazi L, Barbezier N, Depeint F et al. Complexification of in vitro models of intestinal barriers, a true challenge for a more accurate alternative approach. Int J Mol Sci. 2023;10;24:1-33.https://doi.org/10.3390/ijms24043595
9. Herrala M, Huovinen M, Järvelä E, Hellman J, Tolonen P, Lahtela-Kakkonen M, Rysä J. Micro-sized polyethylene particles affect cell viability and oxidative stress responses in human colorectal adenocarcinoma Caco-2 and HT-29 cells. Sci Total Environ. 2023;867:1-9.https://doi.org/10.1016/j.scitotenv.2023
10. Kadac-Czapska K, Ośko J, Knez E, Grembecka M. Microplastics and oxidative stress-current problems and prospects.Antioxidants. 2024;13(5):1-35.https://doi.org/10.3390/antiox13050579
11. Fleury J-B, Baulin VA. Microplastics destabilize lipid membranes by mechanical stretching. Proc Natl Acad. 2021;118(31):1-8.https://doi.org/10.1073/pnas.2104610118
12. Nam TG. Lipid peroxidation and its toxicological implications. Toxicol Res. 2011;27:1-6.https://doi.org/10.5487/TR.2011.27.1.001
13. Malinowska K, Bukowska B, Piwoński I, Foksiński M, Kisielewska A, Zarakowska E et al. Polystyrene nanoparticles: the mechanism of their genotoxicity in human peripheral blood mononuclear cells. Nanotoxicology. 2022;16:791-811.https://doi.org/10.1080/17435390.2022.2149360
14. Paget V, Dekali S, Kortulewski T, Grall R, Gamez C, Blazy K, et al. Specific Uptake and Genotoxicity Induced by Polystyrene Nanobeads with Distinct Surface Chemistry on Human Lung Epithelial Cells and Macrophages. PLoS ONE. 2015;10:1-20.https://doi.org/10.1371/journal.pone.0123297
15. Li L, Lv X, He J, Zhang L, Li B, Zhang X et al. Chronic exposure to polystyrene nanoplastics induces intestinal mechanical and immune barrier dysfunction in mice. Ecotox Environm Safety. 2024;269:1-9.https://doi.org/10.1016/j.ecoenv.2023.115749
16. Gautam R, Jo J, Acharya M, Maharjan A, Lee D, K CP et al. Evaluation of potential toxicity of polyethylene microplastics on human derived cell lines. Sci Total Environ. 2022;838(part2).https://doi.org/10.1016/j.scitotenv.2022.156089
17. Domenech J, Britto M, Velázquez A, Pastor S, Hernández A, Marcos R et al. Long-term effects of polystyrene nanoplastics in human intestinal caco-2 cells. Biomolecules. 2021;11(10):1-16.https://doi.org/10.3390/biom11101442
18. Cortés C, Domenech J, Salazar M, Pastor S, Marcos R, Hernández A. Nanoplastics as a potential environmental health factor: effects of polystyrene nanoparticles on human intestinal epithelial Caco-2 cells. Environ Sci Nano. 2020;7(1):272-85.https://doi.org/10.1039/C9EN00523D
19. Domenech J, Hernández A, Rubio L, Marcos R, Cortés C. Interactions of polystyrene nanoplastics with in vitro models of the intestinal barrier. Arch Toxicol. 2020;94:2997-3012.https://doi.org/10.1007/s00204-020-02805-3
20. Saenen ND, Witters MS, Hantoro I, Tejeda I, Ethirajan A, Van Belleghem F et al. Polystyrene microplastics of varying sizes and shapes induce distinct redox and mitochondrial stress responses in a caco-2 monolayer. Antioxidants. 2023;12(3):1-24.https://doi.org/10.3390/antiox12030739
21. Zeng G, Li J, Wang Y, Su J, Lu Z, Zhang F et al. Polystyrene microplastic-induced oxidative stress triggers intestinal barrier dysfunction via the NF-κB/NLRP3/IL-1β/MCLK pathway.Environ Pollut. 2024;345.https://doi.org/10.1016/j.envpol.2024.123473
22. Wang Q, Bai J, Ning B, Fan L, Sun T, Fang Y et al. Effects of bisphenol A and nanoscale and microscale polystyrene plastic exposure on particle uptake and toxicity in human Caco-2 cells. Chemosphere. 2020;254.https://doi.org/10.1016/j.chemosphere.2020.126788
23. Vecchiotti G, Colafarina S, Aloisi M, Zarivi O, Di Carlo P, Poma A. Genotoxicity and oxidative stress induction by polystyrene nanoparticles in the colorectal cancer cell line HCT116. PLoS One. 2021;16(7):1-18.https://doi.org/10.1371/journal.pone.0255120
24. Wu S, Wu M, Tian D, Qiu L, Li T. Effects of polystyrene microbeads on cytotoxicity and transcriptomic profiles in human Caco-2 cells. Environ Toxicol. 2020;35(4):495-506.https://doi.org/10.1002/tox.22885
25. Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD et al. The PRISMA 2020 statement: an updated guideline for reporting systematic reviews. BMJ. 2021;372:1-9.https://doi.org/10.1136/bmj.n71
26. Schneider K, Schwarz M, Burkholder I, Kopp-Schneider A, Edler L, Kinsner-Ovaskainen A et al.“ToxRTool”, a new tool to assess the reliability of toxicological data. Toxicol Lett. 2009;189(2):138-44.https://doi.org/10.1016/j.toxlet.2009.05.013
27. Paul MB, Böhmert L, Hsiao IL, Braeuning A, Sieg H. Complex intestinal and hepatic in vitro barrier models reveal information on uptake and impact of micro-, submicro- and nanoplastics. Environ Int. 2023;179:1-14.https://doi.org/10.1016/j.envint.2023.108172
28. Visalli G, Facciolà A, Pruiti Ciarello M, De Marco G, Maisano M, Di Pietro A.Acute and sub-chronic effects of microplastics (3 and 10 μm) on the human intestinal cells HT-29.Int J Environ Res Public Health. 2021;18(11):1-12.https://doi.org/10.3390/ijerph18115833
29. Yang Z, DeLoid GM, Baw J, Zarbl H, Demokritou P.Assessment of ingested micro- and nanoplastic (MNP)-mediated genotoxicity in an in vitro model of the small intestinal epithelium (SIE). Nanomaterials. 2024;14(9):1-17.https://doi.org/10.3390/nano14090807
30. Campbell M, McKenzie JE, Sowden A, Katikireddi SV, Brennan SE, Ellis S et al. Synthesis without meta-analysis(SWiM) in systematic reviews: reporting guideline. BMJ 2020;368:1-6. https://doi.org/10.1136/bmj.l6890
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