Revista Fitos
Despite human immunodeficiency virus (HIV) latency still being a key obstacle to permanent cure of infection, several synthetic and natural compounds have already been able to efficiently activate HIV-LTR (long terminal repeat), responsible for re-activating the latent virus. In this study, we report that dichloromethane extract of Jatropha gossypiifolia species (JGDM) has dual effect for inhibition of HIV-1 infection and agonist of protein kinase C (PKC). Using MT-4 cells infected with HIV-1 NL4.3, we found that JGDM is able to decrease HIV-1 replication, with an EC50 of 17.96 μg/mL and promotes reactivation of latent provirus in J-Lat 8.4 and 10.6 cells by 4% and 32%, respectively. In addition, the CD4 expression test showed a 66% reduction of CD4 molecules from surface cells. It was also observed that PKC inhibitor, GÖ6983 inhibited the reactivation and CD4 downregulation activity, suggesting that bioactive compound present in the extract acts through the PKC pathway. In conclusion, the dichloromethane extract of J. gossypiifolia showed dual effect with anti-HIV potential, probably by promoting downregulation of CD4 molecules from cell surface, and potential for reactivating latent HIV-1 through PKC pathway.
DOI
10.32712/2446-4775.2025.1749
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