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Potential of Erigeron bonariensis L. essential oil and its polyacetylene 2-cis-lachnophyllum acid methyl ester as neutralizers of bothropic venoms in vitro

Revista Fitos
Snake envenoming causes human organic multisystem failures and local effects (LEs: hemorrhage, edema and necrose). The antivenom serum is not always effective, generating in extreme cases anaphylaxis and being useless against LEs. An alternative under research is the treatment with alexiteric medicinal plants containing metabolites able to interact and inactivate/neutralize the venom 's components. As, in this context, Erigeron bonariensis L. is a promising species, we aimed to study the alexiteric potential of its essential oil from Uruguay. As assessed by GC/MS, the main components were: 2-cis-lachnophyllum acid methyl ester (cis-LAME,32.8%), 4R-(+)-limonene (22.6%), germacrene D (8.1%), trans-β-ocimene (6.9%) and 1S-(-)-β-pinene (4.8%). cis-LAME was isolated (column chromatography) and identified by spectroscopic methods. The oil and cis-LAME were incubated with Bothrops diporus and B. alternatus venoms, indicating alexiteric potential based on a screening performed by 1D-SDS-PAGE. For B. diporus venom (which causes more envenoming cases in Argentina) were conducted other in vitro tests (inhibition of proteolysis, hemolysis and procoagulant activity). The samples inhibited proteolysis and hemolysis (26.3% and 57.9% for the oil and cis-LAME, respectively), whereas only cis-LAME inhibited procoagulant activity (60.6%). The results obtained confirmed the potential of E. bonariensis to neutralize bothropic venoms, highlighting cis-LAME as a key bioactive component.
DOI
10.32712/2446-4775.2026.1950
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Referências do artigo
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